Consulta: creatorFacets:"Grisolia,Cesar Koppe"
Registros recuperados: 11
Data/hora: 18/05/2024 18:08:18

Association between interleukin 6 -174 G/C promoter gene polymorphism and runners' responses to the dietary ingestion of antioxidant supplementation based on pequi (Caryocar brasiliense Camb.) oil: a before-after study
Provedor de dados: 74
Autores: Miranda-Vilela,Ana Luisa; Ribeiro,Ieler Ferreira; Grisolia,Cesar Koppe.
Abstract Exercise is a double-edged sword: when practiced in moderation, it increases the expression of antioxidant enzymes, but when practiced strenuously it causes oxidative stress and cell damage. In this context, polymorphisms in the interleukin (IL)-6 gene should be investigated better because they can influence performance, at least in exercise that generates oxidative stress and leads to muscular injuries with consequent inflammation. In this work, we investigated the influence of IL-6 –174 G/C polymorphism on tissue damage and inflammation markers, lipid peroxidation, hemogram and lipid profile of runners before and after ingestion of 400 mg of pequi oil in capsules supplied daily for 14 consecutive days. The IL-6 genotypes were associated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant supplementation; Exercise-induced oxidative damage; Inflammatory markers; Nutrigenetics; Nutrigenomics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400554

Comparison between the micronucleus frequencies of kidney and gill erythrocytes in tilapia fish, following mitomycin C treatment
Provedor de dados: 74
Autores: Palhares,Dario; Grisolia,Cesar Koppe.
The frequencies of mitomycin C and cyclophosphamide-induced kidney and gill erythrocyte micronuclei were compared in Tilapia rendalli and Oreochromis niloticus. Cyclophosphamide (CP) and mitomycin C (MMC) were previously used to investigate fish sensitivity to micronuclei induction in kidney erythrocytes. Fish were treated with a single dose of cyclophosphamide (40 mg/kg) or mitomycin C (2 mg/kg). Peripheral blood samples and kidney blood samples were obtained, 1, 2, 3, 5, 7, and 15 days after the intra-abdominal injection of MMC. T. rendalli showed to be more sensitive than O. niloticus to micronuclei production. Both compounds were used at the maximum tolerated dose (MTD), and mitomycin C proved to be more toxic than cyclophosphamide. The frequency of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish micronuclei; Cyclophosphamide; Mitomycin C; Tilapia rendalli; Oreochromis niloticus.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300005

Distribution of glutathione S-transferase GSTM1 and GSTT1 null phenotypes in Brazilian Amerindians
Provedor de dados: 74
Autores: Klautau-Guimarães,Maria de Nazaré; Hiragi,Cássia de Oliveira; D'Ascenção,Renata Ferreira; Oliveira,Silviene Fabiana; Grisolia,Cesar Koppe; Hatagima,Ana; Ferrari,Íris.
The distribution of glutathione S-transferase (GST) GSTM1 and GSTT1 null phenotype frequencies in two Brazilian Amerindian tribes, the Munduruku tribe from Missão Cururu village (79 individuals) and the Kayabi tribe (41 individuals), was analyzed by polymerase chain reaction (PCR) amplification. The GST null phenotype frequencies for the Munduruku sample were 0% for GSTM1 and 27% for GSTT1 while for the Kayabi sample the null phenotype frequencies were 27% for GSTM1 and 29% for GSTT1. This is the first report of the absence of the GSTM1 null phenotype in any ethnic group.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Munduruku; Glutathione S-transferase; Kayabi; Null alleles.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100005

Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant
Provedor de dados: 74
Autores: Grisolia,Cesar Koppe; Oliveira,Adriano Barros Barata de; Bonfim,Helder; Klautau-Guimarães,Maria de Nazaré.
An evaluation of the genotoxicity of domestic sewage was carried out in a municipal wastewater treatment plant. The study was carried out using Allium cepa root tip cells through cytological parameters such as aberrant cells in anaphase-telophase and the mitotic index, and the fish species Oreochromis niloticus and Tilapia rendalli, through the index of micronuclei in peripheral erythrocytes. In the Allium test, each of the four stages of the wasterwater treatment routine was analyzed, i.e., crude sewage, primary effluent, secondary effluent, and tertiary effluent. O. niloticus and T. rendalli specimens were maintained in an aquarium continuously receiving the final effluent, in an open system, before being discharged into Lake Paranoá. For micronuclei...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Biomonitoring; Domestic sewage; Fish; Micronuclei; Mutagenicity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200026

In vitro and in vivo studies demonstrate non-mutagenicity of the herbicide metolachlor
Provedor de dados: 54
Autores: Grisolia,Cesar Koppe; Ferrari,Iris.
The herbicide metolachlor was evaluated for genotoxic potential. Metolachlor did not induce micronuclei in mice, however at 40 mg/kg it significantly decreased the percentage of polychromatic erythrocytes, which is a cytotoxic effect. Metolachlor did not induce chromosomal aberrations in human lymphocytes in vitro, but 2.0 mug/ml culture medium resulted in cytotoxicity, decreasing the mitotic index significantly. The indirect exposure test was carried out by adding plasma from metolachlor-pretreated rats to the human lymphocyte cultures. There was no indication of clastogenicity by metolachlor metabolites. On the other hand, plasma of cyclophosphamide-pretreated rats had a significant clastogenic effect
Tipo: Info:eu-repo/semantics/article
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000300009

Profile of micronucleus frequencies and nuclear abnormalities in different species of electric fishes (Gymnotiformes) from the Eastern Amazon
Provedor de dados: 74
Autores: Melo,Karina Motta; Alves,Ingrid Reale; Pieczarka,Julio Cesar; David,José Augusto de Oliveira; Nagamachi,Cleusa Yoshiko; Grisolia,Cesar Koppe.
The frequency of spontaneous micronucleus (MN) formation in fish species needs to be determined to evaluate their usefulness for genotoxic biomonitoring. The definition of a good bioindicator takes into account the current knowledge of its metabolic traits as well as other factors including its feeding behavior and relationship to the environment. In this study, we compared the basal frequencies of micronucleated erythrocytes and nuclear abnormalities (NA) among different species of the fish Order Gymnotiformes (Rhamphichthys marmoratus, Steatogenys elegans, Sternopygus macrurus, Parapteronotus hasemani, Gymnotus mamiraua, Gymnotus arapaima, Brachyhypopomus beebei, Brachyhypopomus n. sp. BENN) sampled in several localities of the Eastern Amazon. A baseline...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomonitoring; Genotoxicity; Gymnotiformes; Amazon region; Fish erythrocytes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300018

The lipidome, genotoxicity, hematotoxicity and antioxidant properties of andiroba oil from the Brazilian Amazon
Provedor de dados: 74
Autores: Milhomem-Paixão,Susana Suely Rodrigues; Fascineli,Maria Luiza; Roll,Mariana Matos; Longo,João Paulo Figueiró; Azevedo,Ricardo Bentes; Pieczarka,Julio Cesar; Salgado,Hugo Leonardo Crisóstomo; Santos,Alberdan Silva; Grisolia,Cesar Koppe.
Abstract Andirobeira is an Amazonian tree, the seeds of which produce a commercially valuable oil that is used in folk medicine and in the cosmetic industry. Andiroba oil contains components with anti-inflammatory, cicatrizing and insect-repellant actions. However, virtually nothing is known of the safety of this oil for humans. The aim of this work was therefore to investigate the hematotoxicity, genotoxicity and mutagenicity of andiroba oil using the comet and micronucleus assays, and to assess its antioxidant properties and lipidome as a means of addressing safety issues. For the experiments, andiroba oil was administered by gavage for 14 consecutive days in nulliparous female Swiss mice randomly distributed in four groups: negative control and three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute toxicity; Antioxidant activity; Carapa guianensis; Genotoxicity; Lipidomics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200248

The pequi pulp oil (Caryocar brasiliense Camb.) provides protection against aging-related anemia, inflammation and oxidative stress in Swiss mice, especially in females
Provedor de dados: 74
Autores: Roll,Mariana Matos; Miranda-Vilela,Ana Luisa; Longo,João Paulo Figueiró; Agostini-Costa,Tania da Silveira; Grisolia,Cesar Koppe.
Abstract Continued exposure to reactive oxygen species and inflammation are the rationale behind aging theories and associated diseases. Scientific evidence corroborates the ethnomedicinal use of the oil of pequi (Caryocar brasiliense Camb.), a typical Brazilian Cerrado fruit, against oxidative damage to biomolecules and inflammation. We aimed to investigate in vivo the antioxidant and anti-inflammatory effects of pequi oil on hemogram and DNA damage in healthy young adult and older middle-aged Swiss mice of both genders. Animals, aged 6-7 and 11-12 months, were orally treated for 15 days with pequi oil at 30 mg/day. Blood samples were used for hemogram and comet assay, and bone marrow for micronucleus test. Female controls of 11-12 months had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging-related inflammation and oxidative stress; Comet assay; Micronucleus; Antioxidant; Folk medicine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500858

Toxicity and genotoxicity in Astyanax bimaculatus (Characidae) induced by microcystins from a bloom of Microcystis spp
Provedor de dados: 74
Autores: Silva,Ricardo Rocha Pavan da; Pires Junior,Osmindo Rodrigues; Grisolia,Cesar Koppe.
Studies of genotoxicity in fish caused by cyanobacterial microcystins can be useful both in determining the sensitivity of native species, as well as comparing exposure routes. The genotoxicity caused by the microcystins LR and LA from a bloom collected in a eutrophic lake, was revealed in the fish Astyanax bimaculatus, a native species from South America. LC50 (72 h) was determined as 242.81 µg L-1 and LD50 (72 h) as 49.19 µg kg-1 bw. There was a significant increase of DNA damage in peripheral erythrocytes, following intraperitoneal injection (ip) with tested concentrations of 24.58 µg kg-1 bw and 36.88 µg kg-1 bw, as well as through body exposure to a concentration of 103.72 µg L-1. Micronucleus (MN) induction was observed after ip injections of 24.58...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcystin; Comet assay; Fish micronucleus; Necrosis; Apoptosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400023

Variability in micronucleus induction with different mutagens applied to several species of fish
Provedor de dados: 74
Autores: Grisolia,Cesar Koppe; Cordeiro,Célia Maria Torres.
Fish are often used for screening genotoxicity of water. For such programs, a knowledge of the sensitivity to clastogens, spontaneous micronucleus frequency and cell cycle kinetics of the target tissue is necessary. To investigate the pattern of inter-specific sensitivity to micronucleus induction three species of fish, Tilapia rendalli, Oreochromis niloticus and Cyprinus carpio, were exposed to the clastogens bleomycin (BLM), cyclophosphamide (CP), 5-fluorouracil (5-FU), and mitomycin C (MMC). The binucleate/mononucleate ratio in peripheral erythrocytes exposed to cytochalasin B was also used to evaluate the time-dependent response of micronucleus formation during hematopoesis in the kidney and the micronucleus peak in peripheral erythrocytes....
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100041

β-globin haplotypes in normal and hemoglobinopathic individuals from Reconcavo Baiano, State of Bahia, Brazil
Provedor de dados: 74
Autores: Silva,Wellington dos Santos; Klautau-Guimarães,Maria de Nazaré; Grisolia,Cesar Koppe.
Five restriction site polymorphisms in the β-globin gene cluster (HincII-5'ε, HindIII-Gγ, HindIII-ªγ, HincII-'ψβ1 and HincII-3''ψβ1) were analyzed in three populations (n = 114) from Reconcavo Baiano, State of Bahia, Brazil. The groups included two urban populations from the towns of Cachoeira and Maragojipe and one rural Afro-descendant population, known as the "quilombo community", from Cachoeira municipality. The number of haplotypes found in the populations ranged from 10 to 13, which indicated higher diversity than in the parental populations. The haplotypes 2 (+----),3(----+),4(-+--+)and6(-++-+)onthe βA chromosomes were the most common, and two haplotypes, 9 (-++++)and 14 (++--+), were found exclusively in the Maragojipe population. The other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Afro-descendant; Β-globin; Haplotypes; Hemoglobinopathies.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300002